Restricted Research - Award List, Note/Discussion Page

Fiscal Year: 2021

244  University of North Texas  (84540)

Principal Investigator: Henard,Calvin A

Total Amount of Contract, Award, or Gift (Annual before 2011): $ 7,347

Exceeds $250,000 (Is it flagged?): No

Start and End Dates: - 3/31/22

Restricted Research: YES

Academic Discipline: Biological Sciences

Department, Center, School, or Institute: College of Science

Title of Contract, Award, or Gift: Molecular Coliform Testing

Name of Granting or Contracting Agency/Entity: Texas Green Star Holdings, LLC


Program Title: N/A


The Henard laboratory will develop methodology to quantify total coliform and total Escherichia coli in water using quantitative polymerase chain reaction (qPCR). This method will be used to analyze water samples (up to 250) collected and provided to UNT by Texas Green Star at a frequency of ~20 samples/month. Specifically, total DNA will be isolated from samples and used as template in a multiplex qPCR reaction containing coliform (lacZ)- and E. coli (yaiO)-specific primers/probes. Absolute coliform and E. coli numbers will be calculating via regression analysis by comparing to a standard curve from known DNA/bacteria concentration. The Henard laboratory will provide a sample submission form for use by Texas Green Star to facilitate sample tracking and inventory. The Henard lab will maintain DNA samples at -20°C for at least 2 years from the date of isolation. DELIVERABLES The Henard laboratory will provide raw (Ct values) and transformed (CFU coliform/100mL water; CFU E.coli/100mL water) data in a spreadsheet to Texas Green Star within 24 h of sample submission.

Discussion: No discussion notes


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