Restricted Research - Award List, Note/Discussion Page

Fiscal Year: 2021

200  University of North Texas  (84496)

Principal Investigator: Root,Douglas Donald

Total Amount of Contract, Award, or Gift (Annual before 2011): $ 262,511

Exceeds $250,000 (Is it flagged?): Yes

Start and End Dates: - 7/31/24

Restricted Research: YES

Academic Discipline: Biological Sciences

Department, Center, School, or Institute: College of Science

Title of Contract, Award, or Gift: Length-Dependent Activation in Human Myocardium

Name of Granting or Contracting Agency/Entity: University of Kentucky

Program Title: N/A
CFDA Linked: Heart and Vascular Diseases Research


1.1.1 (SAM); IACUC Protocol Application Number: 19-014; Human Subjects Application No. 18-492; This scope of work will address multiple aims with the greatest emphasis on Aim 3: Modulate length-dependent activation in myocardium from patients who have heart failure. Specifically, the design and characterization of synthetic peptides for modulating length-dependent activation in human myocardium will be delivered. OBJECTIVES:1. Peptides will be designed to modulate myosin subfragment-2 (S2) structural stability using molecular mechanics and dynamics computational chemistry software in order to affect the binding of myosin’s interacting heads motif to the S2.2. The designed peptides will be synthesized, fluorescently labeled, modified for cell membrane penetration, and purified in sufficient quantities to be characterized and shared with Dr. Tanner’s laboratory for studies on human cardiac muscle mechanics.3. The synthesized peptides will be characterized for binding affinity, impact on coiled coil structure, and binding specificity within the sarcomere of myofibrils. The data will be collected from human and mouse models; although, some of the preliminary data was collected using rabbits.

Discussion: No discussion notes


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