Restricted Research - Award List, Note/Discussion Page

Fiscal Year: 2021

131  University of North Texas  (84427)

Principal Investigator: Chapman,Kent D

Total Amount of Contract, Award, or Gift (Annual before 2011): $ 115,000

Exceeds $250,000 (Is it flagged?): No

Start and End Dates: - 12/31/21

Restricted Research: YES

Academic Discipline: Biological Sciences

Department, Center, School, or Institute: College of Science

Title of Contract, Award, or Gift: Engineering Seed Value in Cotton - Strategies to Modify Seed Constituents

Name of Granting or Contracting Agency/Entity: Cotton Incorporated


Program Title: N/A


The long-term goal is to develop traits in cotton plants for added seed value. The focus of our work is to understand the relationships between seed oil, protein and fiber characteristics, so that genetic and/or environmental factors can be identified that facilitate the manipulation of seed components for maximum economic benefit. Efforts will be focused this year on quantifying the natural variation in seed protein and oil in existing cotton breeder materials. Non-destructive, time-domain 1H-NMR analyses will be scaled up to support the measurements of seed protein and oil content in Cotton Incorporated- sponsored research programs. Capturing the variation in seed quality traits in combination with fiber traits and seedling vigor evaluations will provide new information for selection of improved varieties for growers. Samples from selected sites in the cotton regional breeder testing network (RBTN) will be coordinated through Don Jones at Cotton Incorporated to obtain measurements of seed oil content, protein content and associated fiber quantity and quality factors. In addition, other studies will be supported with seed analyses including irrigation trials by the USDA-ARS, and the Beltwide Seed Quality project coordinated by Gaylon Morgan at Cotton Inc. The availability of non-destructive measurements of seed oil and protein content could also be directed to future studies associated with planting seed quality as this becomes a greater focus of the cotton industry. In addition, to evaluating the general storage components in the embryo, specific changes in seed characteristics have been targeted for development by both transgenic and non-transgenic approaches. We identified non-transgenic alleles for elevated seed oleic acid content in a wild accession of Gossypium barbadense, and in cooperation with Lori Hinze, USDA-ARS, these alleles are being transferred to adapted varieties. In addition, efforts continue in collaboration with Monica Schmidt at the University of Arizona, to introduce RNAi suppression constructs to block aflatoxin production (Thakare D et al., 2017, Science Advances 3(3) e1602382) in cottonseeds, and numerous transgenic events are being transferred to her program for testing. Recently, vitamin E profiles were altered in cottonseed through transgenic means, and feeding studies have been scheduled with these new enhanced oils. Finally, a new collaboration with Jay Shockey at the USDA-ARS is aimed at manipulating the accumulation of cyclic fatty acids in cottonseeds by targeting the expression of cyclopropane fatty acid synthase (CPS).

Discussion: No discussion notes


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